Journal: STAR Protocols

Article Title: In vitro and in cellulo ApoE particle formation, isolation, and characterization

doi: 10.1016/j.xpro.2022.101894

Figure Lengend Snippet: Lipid separation and detection by HPTLC (A) HPTLC plate preparation and automated lipid spotting. The HPTLC plate is prewashed with an excess of chloroform:methanol 1:1 (v/v) and dried briefly. The plate is placed in the automated HPTLC sampler (CAMAG ATS4) and lipid extracts and standards are spotted. (B) Lipid separation. The HPTLC plate from (A) is developed in a first solvent system (chloroform:methanol:ammonium hydroxide 65:25:4 (v/v/v)) for 5 cm and dried briefly. Next, the plate is developed in the second solvent system (hexane:diethyl ether:acetic acid 80:20:2 (v/v/v)) that is left to migrate for 9 cm. Finally, the plate is dried under vacuum for 30 min at 20°C–25°C. Note : The first solvent system separates hydrophilic lipids like phospholipids (black spots) and the second solvent system separates hydrophobic lipid species like cholesterol, triacylglycerols and cholesteryl esters (blue spots). At this step, lipids are not visible - the dots were represented in the scheme to depict the lipid separation pattern. (C) Lipid revelation. After lipid separation, the HPTLC plate is stained with a CuSO 4 staining solution for 1 min. The excess solution is decanted and the plate is dried under vacuum for 15 min at 20°C–25°C. Lipids are charred at 145°C for 7.5 min and the plate is visualised in visible light or fluorescence at 488 nm or 546 nm. See also . Created with BioRender.com .

Article Snippet: The plate is placed in the automated HPTLC sampler (CAMAG ATS4) and lipid extracts and standards are spotted. (B) Lipid separation.

Techniques: High Performance Thin Layer Chromatography, Solvent, Staining, Fluorescence

Journal: STAR Protocols

Article Title: In vitro and in cellulo ApoE particle formation, isolation, and characterization

doi: 10.1016/j.xpro.2022.101894

Figure Lengend Snippet:

Article Snippet: The plate is placed in the automated HPTLC sampler (CAMAG ATS4) and lipid extracts and standards are spotted. (B) Lipid separation.

Techniques: Recombinant, Modification, Enzyme-linked Immunosorbent Assay, Software, Blocking Assay, Membrane, Imaging, High Performance Thin Layer Chromatography

Journal: Analytical and Bioanalytical Chemistry

Article Title: Development of a targeted HPLC-ESI-QqQ-MS/MS method for the quantification of sulfolipids from a cyanobacterium, selected leafy vegetables, and a microalgae species

doi: 10.1007/s00216-021-03164-3

Figure Lengend Snippet: Fig. 3 HPTLC of lipid extracts from Arthrospira sp. extracted with different solvents (according to Table 2). Derivatization was performed with 5% copper sulfate in 8% phosphoric acid and then heated for 30 min at 160 °C

Article Snippet: The samples were applied as bands (8 mm) using an HPTLC autosampler (ATS4, CAMAG AG, Muttenz, Switzerland).

Techniques: High Performance Thin Layer Chromatography